Journal: Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research

Article Title: Skeletal colonization by breast cancer cells is stimulated by an osteoblast and β2AR-dependent neo-angiogenic switch

doi: 10.1002/jbmr.3133

Figure Lengend Snippet: A) Representative 40× H&E images of hind limb bone sections from athymic nude mice treated with PBS, 3mg/kg Isoproterenol (ISO), or Chronic Immobilization Stress (CIS). Blood vessels can be recognized by their shape and staining of red blood cells (red). Bar: 100 μm. B) Quantification of vessel area (VsA) and vessel number normalized by tissue area (TA) in mice that received PBS (N=12) or ISO (N=7) (p=.009 and p=.037), as well as mice subjected to control (N=5) or CIS (N=6) (p=.296 and p=.004). C) Quantification of primary mouse BMEC tube length (*=p<.05, N=3). D) Quantification of HUVECs tube length (*p=<.05, ***=p<.001, N=6). E) Schematic of the metatarsal assay and representative 4× Images of metatarsals according to each treatment. After 2 weeks of culture, mouse explants were stained for the endothelial cell marker CD31. F) Quantification of the number of CD31+ parental vessels sprouting from the metatarsal bones (*P=<.05, ****P=<.0001, N≥3).

Article Snippet: Twenty-four well plates were coated with 200 μL of growth factor reduced matrigel (Corning #354230) for 45 mins. at 37°C before 30,000 HUVECs (Gibco #C0035C), or BMECs from wild-type C57BL/6 mice were plated and cultured in 500 μL of Basal Endothelial Cell Growth Media (ScienCell #1001).

Techniques: Staining, Control, Marker